免疫原Recombinant IL33 (Ser112~Thr228) expressed in E.coli
基因ID90865
蛋白编码O95760
同义词DVS27-related protein; H-IL-33; IL-1F11; IL-33; ILN; Interleukin; Interleukin-1 family member 11; Interleukin-33; Interleukin33; NF-HEV; nuclear factor for high endothelial venules; Nuclear factor from high endothelial venules; RP11-575C20.2
宿主物种Mouse
抗体分型IgG2a Kappa
纯化Affinity purification
储存Store at -20℃. Avoid freeze / thaw cycles.
靶点背景IL-33 (Interleukin-33) is a 270 amino acid, highly divergent protein belonging to the IL-1 family with an IL-1-like C-terminal domain. IL-33 is a dual function protein that may function both as a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. IL-33 binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6. IL-33 activates NF-kappaB and MAP kinases, and drives production of TH2-associated cytokines from in vitro polarized TH2 cells. In vivo, IL-33 induces the expression of IL-4, IL-5, and IL-13 and leads to severe pathological changes in mucosal organs. IL-33 is proteolytically converted to a mature form by CASP1 and is highly expressed in high endothelial venules found in tonsils, Peyer's patches and mesenteric lymph nodes and is almost undetectable in placenta. Prolonged IL-33 treatment of mice led to the development of eosinophilia, splenomegaly, and severe pathological changes in mucosal organs such as lungs, esophagus and small intestine. Recent experiments have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. Despite its predicted molecular weight, IL-33 will often run at higher molecular weight in SDS-PAGE. Studies have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine, and an intracellular nuclear factor with transcriptional regulatory properties.
推荐稀释比WB 1:1000-1:2000 IHC-P 1:50-1:200
产品形式Liquid
Buffer 体系PBS with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
