Product Information： | |
Product name | VEGFA Polyclonal Antibody |
Isotype | IgG |
Form | Lyophilized or Liquid |
Tested applications | WB,IHC,IF,ELISA |
Species reactivity | Human,Mouse,Rat |
Host Species | Rabbit |
Purity | Affinity purification |
Immunogen Information：
Immunogen | Recombinant protein of human VEGFA |
Calculated molecular weight | 15-27kDa/34-45kDa |
Observed molecular weight | 42kDa |
Swiss Prot | P15692 |
Gene ID (NCBI) | 7422 |
Alternative Names | VEGFA; MVCD1; VEGF; VPF; vascular endothelial growth factor A |
Application Notes：
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB | 1:500 - 1:2000 |
IHC | 1:50 - 1:200 |
IF | 1:50 - 1:200 |
Background：
This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. Allelic variants of this gene have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site.
Storage：
Storage instructions | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Services & Support：
1. Please do not hesitate to contact us if you have any questions.
2. Please fill in the Technical Suppoprt Request Form and email the form to jiyinmei027@163.com within 3 months of receipt of the goods, and we will response to your inquiry within 24 hours.
3. The after-sale service channel will be shut down after 3 months.
