Product Information： | |
Product name | MAP3K5 Polyclonal Antibody |
Isotype | IgG |
Form | Lyophilized or Liquid |
Tested applications | WB,ELISA |
Species reactivity | Human,Mouse,Rat |
Host Species | Rabbit |
Purity | Affinity purification |
Immunogen Information：
Immunogen | A synthetic peptide of human MAP3K5 |
Calculated molecular weight | 69kDa/154kDa |
Observed molecular weight | 154kDa |
Swiss Prot | Q99683 |
Gene ID (NCBI) | 4217 |
Alternative Names | ASK1;MAPKKK5;MEKK5 |
Application Notes：
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB | 1:500 - 1:2000 |
Background：
Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK.
Storage：
Storage instructions | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Services & Support：
1. Please do not hesitate to contact us if you have any questions.
2. Please fill in the Technical Suppoprt Request Form and email the form to jiyinmei027@163.com within 3 months of receipt of the goods, and we will response to your inquiry within 24 hours.
3. The after-sale service channel will be shut down after 3 months.
